Van Keer et al, 2021
Aim: To study the feasibility of testing six high-risk HPV DNA methylation markers in first-void urine to distinguish underlying high-grade cervical disease from normal tissue and low-grade lesions in women diagnosed with high-risk HPV infection.
Affiliations: University Hospital Antwerp (UZA), University Antwerp (UA), Vrije Universiteit Amsterdam Pathology Cancer Centre (UMC)
Summary: The study results show the feasibility of first-void urine for the analysis of DNA methylation of the six selected HPV host-cell genes analyzed in this study. Therefore, first-void is suggested to have diagnostic potential to distinguish normal/low-grade from high-grade underlying cervical disease in high risk-HPV infected women. Concomitantly, urine can be considered a promising liquid biopsy that offers a fully molecular screen and triage strategy based on primary HPV testing and methylation marker detection in the same sample.