Optimization of HPV DNA detection in urine by improving collection, storage, and extraction

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Vorsters et al., 2015

Aim: To examine the impact of a DNA-conservation buffer, the extraction method, and urine sampling on the detection of HPV DNA and human DNA in urine provided by 44 women with a cytologically normal but HPV DNA-positive cervical sample.

Affiliations: Centre for the Evaluation of Vaccination (CEV), the Laboratory of Experimental Hematology and the Laboratory Microbiology of the Vaccine & Infectious Disease Institute (VAXINFECTIO) and the Applied Molecular Biology Research Group at the University of Antwerp; Department of Clinical Microbiology of the Antwerp University Hospital

Summary: The results of this study showed that an optimized method for HPV DNA detection in urine should (a) prevent DNA degradation during extraction and storage, (b) recover cell-free HPV DNA in addition to cell-associated DNA, (c) process a sufficient volume of urine, and (d) use a first-void sample.

Optimization of HPV DNA detection in urine by improving collection, storage, and extraction