Impact of Collection Volume and DNA Extraction Method on the Detection of Biomarkers and HPV DNA in First-Void Urine

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Téblick et al., 2021

Aim: To investigate whether different first-void urine volumes (4, 10 and 20mL) affects the analytical detection of human and viral endpoints and to evaluate different HPV DNA extraction protocols.

Affiliations: University Hospital Antwerp (UZA), University of Antwerp, Novosanis NV, Self-Screen B.V, 1098 RX Amsterdam, The Netherlands, UMC, Pathology, Cancer Center Amsterdam

Summary: A good agreement between the three different first-void urine collection volumes was found for all human endpoints. The authors concluded a preference for the Colli-Pee® device allowing a 10 mL first-void urine collection volume because it facilitates high-throughput screening. In addition, Téblick et. al., report a clear HPV DNA extraction protocol taking into account financial costs and laboratory labor intensiveness.

Impact of Collection Volume and DNA Extraction Method on the Detection of Biomarkers and HPV DNA in First-Void Urine