HPV testing in first-void urine provides sensitivity for CIN2+ detection comparable to a physician-taken smear or brush-based self-sample: cross-sectional data from a triage population
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Leeman et al., 2017
Aim: To determine the sensitivity and specificity of high-risk HPV (hrHPV) detection in self-collected urine samples collected in the morning and later during the day, vaginal brush-based self-samples and clinician-taken smears for detecting cervical cancer lesions in a Spanish colposcopy referral population.
Affiliations: DDL Diagnostic Laboratory, University of Barcelona, Universtity Hospital Barcelona, Erasmus Medical Cente Rotterdam
Summary: The study revealed a substantial to almost excellent agreement between all sample types for hrHPV detection. In addition, first-void morning urine collection was found to be convenient and to have a similar CIN2+ detection sensitivity compared to vaginal self-samples and clinicians taken smears. Furthermore, these results show that there is no advantage in using first-void urine collected during the morning over a portion of first-void urine collected at a later time during the day for hrHPV testing.
Additionally, the following posters below report the interim results that led to the publication:
1. First-void urine and physician-taken smear show similar sensitivity for the detection of CIN2+ lesions
Leeman et al, ECCMID Amsterdam, 2016 (Poster)
Aim: Compare self-collected urine, brush-based self-samples (SS) and physician-taken smears (PTS) for HPV detection and variation between morning collection (U1) and collection during the day (U2).
Affiliations: DDL Diagnostic Laboratory (The Netherlands), Faculty of Medicine - University of Barcelona (Spain)
Results: CIN2+ detection using HPV testing in first-void urine is feasible, with sensitivity similar to the sensitivity and specificity to PTS and SS. No advantage in testing morning first-void urine over first-void from later during the day was observed.
2. The detection of hrHPV-infection of the cervix using first-void urine in women with an abnormal pap-smear
Leeman et al, International Papillomavirus Conference, Lisbon, 2015 (Poster)
Aim: Determine the sensitivity and specificity of morning first-void urine for HPV detection compared to first-void urine from later during the day and to study the correlation between HPV detection using self-collected urine samples and physician-taken cervical smears.
Affiliations: DDL Diagnostic Laboratory (The Netherlands), Faculty of Medicine - University of Barcelona (Spain)
Results: Detection of CIN2+ lesions through HPV-testing in first-void urine seems feasible, with a sensitivity and specificity similar to the physician-taken smears. The data suggest that there is no advantage in testing morning first-void urine over first-void from later during the day.
