Optimization of HPV DNA detection in urine by improving collection, storage, and extraction

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Vorsters et al, Eur J Clin Microbiol Infect Dis 2015 (Publication)

Aim: Evaluate the effects of storage, sample preparation, extraction, and sampling on the detection of HPV DNA in the urine of HPV-positive women

Partners: Centre for the Evaluation of Vaccination, Vaccine & Infectious Disease Institute, University of Antwerp (Belgium)

Results: An optimized method for HPV DNA detection in urine should (a) avoid DNA degradation, not only during storage but also during extraction, (b) recover cell-free HPV DNA in addition to cell-associated DNA, (c) process a sufficient volume of urine, as the concentration of HPV DNA may be limited, and (d) use first-void urine