Human papillomavirus detection in urine: Effect of a first-void urine collection device and timing of collection

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Pattyn et al, J Virol Methods 2019 (Publication)

Aim: The effect of a first generation first-void (FV) urine collection device and variation between morning collection and collection during the day.

Partners: University of Antwerp, VAXINFECTO (Belgium), I-BIOSTAT, LMM

Results: The results provide further evidence that when an appropriate preservative and DNA extraction method in combination with a PCR-based HPV test is used, FV urine is a reliable sample for HPV DNA testing. FV urine collected in the morning did not contain a higher HPV DNA concentration than FV urine collected later during the day. Colli-Pee® collected samples showed higher HPV concentrations than cup collected samples.

https://www.ncbi.nlm.nih.gov/pubmed/30452931

 

Additionally, the following posters report the interim results that led to the publication:

1. HPV DNA detection in urine: effect of a first-void urine collection device and timing of collection

Vorsters et al, International Papillomavirus Conference Lisbon, 2015 (Poster)

Aim: Evaluate the use of a prototype first-void (FV) urine collection device Colli-Pee® and to assess the effect of time of collection on detection of human and HPV DNA in women.

Partners: University of Antwerp, VAXINFECTIO, Product Development (Belgium)

Results: With an appropriate preservative and DNA extraction method a reliable and reproducible urine sample for HPV DNA testing, independent of whether a FV urine sample is taken at the start of the day or later in the day can be captured.

2. HPV DNA detection in urine: effect of a first-void urine collection device and timing of collection

Pattyn et al, International Papillomavirus Conference Cape Town, 2017 (Poster)

Aim: Investigate the effect of a first-void self-collection urine device to detect HPV DNA and to assess if the concentration of HPV DNA varies between urine collected in the morning versus later in the day.

Partners: University of Antwerp, VAXINFECTO (Belgium), University Hospital Antwerp (Belgium)

Results: With an appropriate preservative and DNA extraction method , FV urine is a reliable and reproducible sample for HPV DNA testing, independent of whether the FV urine is taken from the first urine of the day or from urine provided later in the day.