Vanlerberghe et al, Eurogin Lisbon, 2018 (Poster)
Aim: Develop a protocol for DNA extraction from first-void urine (FVU) and to evaluate the performance with the INNO-LiPA® HPV Genotyping Extra II compared to cervical samples.
Partners: Fujirebio, University of Antwerp, VAXINFECTO (Belgium), Department of Medicine and Surgery, University of Milano-Bococca, Monza
Results: Initial feasibility data demonstrate that the INNO-LiPA® HPV Genotyping Extra II assay can be used on UCM-preserved FVU. HPV DNA genotyping on paired cervical and FVU samples resulted in > 95% concordant results. The high analytical sensitivity of the assay allows reduction of the sample volume and could eliminate the need for a concentration step before extraction. The obtained data suggest that with optimization and standardization of the pre-analytical handling protocol almost equivalent performance of the INNO-LiPA® HPV Genotyping Extra II can be achieved both on cervical and FVU samples.